Showing posts with label CBSE. Show all posts
Showing posts with label CBSE. Show all posts

Tuesday 26 February 2019

REPRODUCTION IN ORGANISMS: Key Points


Life Span: Period frm birth to natural death
Life span not related to size (e.g. mango – short; peepal – long)
Reproduction significance: Continuity of species
Factors on which mode of reproduction depends:
 habitat, internal physiology etc.
Cell div mode of rep in unicellular org.
In favourable condition –
Binary fission: In amoeba & paramecium (two equal halves)
In Unfav conditions:
Encystation & Sporulation
(formation of minute Amoeba or Pseudopodiospores)
Budding: yeast (2 unequal halves)
In Fungi & Algae: Asexual rep through spores:
Types:
Zoospores – motile; zygospores – non motile
Conidia – in Penicillium; Gemmules – in Sponges
Fragmentation – Hydra
Water Hyacinth (terror of Bengal), high rate of veg propagation.
Veg propagules: Potato: Buds (eyes), Banana & Ginger: Rhizome; 
Bryophyllum: Adventitious buds on leaf margins…… Key feature: NODE
In simple org: asexual rep in fav conditions;
sexual in unfav (provides variations, enables protection by hard seed coat)
In higher org: sexual rep common, asex rare. In animals only sexual
Sexual Rep: Elaborate, Complex & Slow. Offspring not identical to parents
Common pattern of sexual rep:
  • Complete juvenile/vegetative (in plants) phase
  • Beginning of rep phase (flowering in plants)
  • senescent phase
Length of the 3 phases variable in different organisms
Hormones responsible for transition between 3 phases.
Unique: Bamboo perennial but flowering once in lifetime
Strobilanthus kunthiana once in 12 yrs.
Animals: e.g. birds seasonal breeders in nature (in captivity; exploited)
Placental Mammals: Cyclical changes in ovaries & Hormones
Non primate mammals: Oestrous Cycle
Primate mammals: Menstrual cycle
Events: Pre-fertilization, Fertilization & Post fertilization



Gametogenesis: Male & Female, haploid, may be homogametes (isogametes) or heterogametes.
Male: antherozoid or sperm; female: egg or ovum
Sexuality in plants: Unisexual/Dioecious/Heterothallic e.g. papaya, date palm
OR Bisexual/Monoecious/Homothallic
Male: staminate; Female: Pistillate
If make and female flower on different plants: dioecious
If male & female flower on same plant: Monoecious
Sexuality in animals:
Bisexual (Hermaphrodite): Earthworm, Sponges, tapeworm & leech
Unisexual: Rest
Gamete Formation: Haploid
Parents body may be
haploid: e.g. fungi, algae, Bryophytes. Produce gametes by mitosis
Diploid: e.g. pteridophytes, Gymnosperms, Angiosperms and all animals.
Produce gametes by Meiosis
Site of meiosis in diploid organisms: Meiocytes (gamete mother cells)
Gamete Transfer: organisms where both male & female gametes: fungi & Algae
To compensate for loss during transfer, male gametes produced in large numbers.
In bisexual flowers – self fertilization
In unisexual flowers – cross fertilization, so pollination important.
Fertilisation/ Syngamy:
Dev of new organisms without fertilization : Parthenogenesis
Seen in Rotifers, Honeybees & some Lizards.
Site of Fertilization: Outside (External), Inside mother’s body (Internal) fertilization.
Disadvantages of Ext Fertilization: Gametes can be destroyed by predators.
Technique to avoid this – large number of gametes released.
Post Fertilization Events: Formed in medium (external fert)/ In Mother’s body (Int Fert)
Types of development;
  • Undergoes period of rest- fungi & Algae
  •  (develops thick wall to protect from desiccation and damage)
  • Undergoes Meiosis – Haplontic life cycle – forms haploid spores
  • Forms embryo
Embryogenesis: Zygote                  Cell Division & Cell Differentiation                                                         Embryo
Oviparous vs Viviparous

Parts of flower after fertilization:
Petals: wither & fall
Sepals: Wither and Fall (Exception- Brinjal, Tomato)
Pistil: Remains attached
Stamen: wither & Fall
Ovary: Fruit (Wall of fruit – Pericarp)






And now some questions:

  1. Why unicellular organisms are considered immortal?
  2. Why progeny of asexual reproduction called Clones?
  3. What are vegetative Propagules?
  4. Characterize: Veg, rep & senescent phase in annual, biennial & Perennials
  5. Differentiate: Seasonal & Continuous Breeders

Thursday 21 February 2019

BIOTECHNOLOGY

IMPORTANT TOPICS

BOARD PREPARATION


  1. Palindromes with e.g. & diagram
  2. Endonuclease vs. exonuclease
  3. Restriction endonucleases, nomenclature
  4. Cloning vectors, characteristics & diagram
  5. Isolation of DNA, Enzymes
  6. Gel Electrophoresis, Principle, Technique
  7. Modes of Transformation (Ti, Competence, Microinjection, Gene Gun)
  8. PCR
  9. Types of Bioreactors, Downstream Processing
  10. Selection (Antibiotic: Replica Plating; Insertional Inactivation)
  11. Bt, cry
  12. Insulin Production
  13. RNA interference, ADA deficiency
  14. Bioethics, Biopatents
  15. GEAC

Saturday 9 February 2019

Key Points: Evolution (Big Bang & Origin) Part 1


 

XII

         

EVOLUTION

QUESTIONS

NOTES


Age of universe: 20 bn yrs ago
Age of Earth: 4.5 bn yrs ago
Life appeared: 4 bn yrs ago

Universe m/o clusters of galaxy. Galaxy m/o clusters of stars, clouds of gases & dust.
BIG BANG THEORY: explains origin of universe.

Composition of Earth: Water vapour, methane, CO2, NH3 (frm molten mass on Earth’s surface)
No atmosphere

Processes on Earth post big bang led to formation of CO2, water etc.

Theories of Origin of Life:
·       Panspermia: Life came from outer space as ‘spores’
·       Spontaneous generation: Life originated from decaying matter
·       Life comes from pre-existing life – Louis Pasteur
·       Abiological Origin of life: Oparin & Haldane
·       Exp demo of above: Miller
Define Biogenesis
Biogenesis/ Chemical Evolution:
Inorganic molecules            Non-living organic molecules s/a proteins, RNA etc.                                                   Life
Conditions required: High temp, Volcanic storms, reducing atmosphere containing CH4, NH3 etc

Miller’s exp:
Flask with: CH4, NH3, H2 (red. Atm)
                     Water vapour
                     Electric Spark (high temp – 800°C)
Result: Amino acids formed
Further Proof:
·       Others obtained sugars, pigments, fats, N bases in similar exp
·       Meteorite content reveals similar material from other places in space



Why DNA is preferred Genetic Material

Genetic material should be capable of:
  1. Stability: storing genetic information, chemical & Structural stability
  2. Expression: able to express in from of traits or 'Mendelian Characters'
  3. Replication: Be able to duplicate genetic material accurately
  4. Inheritance: pass on copies of genetic information to next generation 
  5. Evolution: allow production of variations through mutation or recombination
DNA vs RNA as genetic material

Both DNA and RNA have ability to act as genetic material but RNA preferred because:

  1. DNA has Deoxyribose while RNA has Ribose - Chemical stability
  2. Thymine in DNA while Uracil In RNA - Chemical stability
Chemically less reactive
➤Stability proved by Transformation (Griffith's experiment)
  1. DNA double stranded, RNA single stranded - structural stability
  2. Ability to replicate: complementary base pairing in dsDNA allows accurate copying during semiconservative replication 


Both DNA & RNA can express themselves through proteins. In fact protein synthesis cannot occur without RNA.
Ability to undergo mutation: both DNA & RNA able to mutate. In fact RNA mutates faster than DNA.

RNA being more reactive, and DNA being more stable; DNA was preferred over RNA as genetic material.


Now having read the topic try answering these questions:
  1. Why is RNA more suitable in a catalytic role?
  2. It is difficult to develop vaccines against RNA viruses s/a Rhinovirus (common cold Virus) or HIV?  
  3. Justify, RNA is better suited for transmission of genetic information.

Tuesday 16 January 2018

Biotechnology & Its Applications: Key points

 

         

 Name: ________________________________________
Class:  ______________________     Topic:  BIOTECHNOLOGY & ITS APPLICATIONS
Date:   _______/ _____/ ________                                         
Period ______                  

QUESTIONS

NOTES


AGRICULTURE

  1. Bt: Bacillus thuringiensis

Bacillus thuringiensis produces a protein that kills certain insects: lepidopterans (tobacco budworm, army worm), coleopterans (beetles), dipterans (flies, mosquito)

Bacillus thuringiensis form protein crystals that are insecticidal

In Bacillus – present as inactive protein – protoxin

Inside insect gut, at alkaline pH, gets converted to active toxin

Active toxin binds to surface of epithelial lining of insect gut, forms pores, causes swelling, cells lysis, death of insect.

Genes for Bt toxin cry – 3 types:
Cry IAc & cry II Ab for cotton bollworms
Cry I Ab for corn borer

The gene introduced in cotton using Ti plasmid


  1. RNA interference: production of pest resistant plants – against nematodes

Meloidegyne incognita (Nematode) infects roots of tobacco & damages plants

RNA interference – not allowing expression of mRNA by creating a dsRNA

dsRNA can be made by making a complementary strand of RNA, which binds to mRNA – RNA silencing
What are transposons?
Genes that are able to chang their position, replicate through RNA intermediate
In our body it occurs as a method of cellular defense – introduced by infecting viruses or transposons (jumping genes)

To control nematodes infection – nematode genes introduced in tobacco (host)

Host makes both sense antisense RNA for these genes

The 2 RNAs being complementary bind to nematode RNA & do not allow it to express – silencing

Nematode not able to survive in host



MEDICINE

  1. Genetically engineered Insulin – humulin

Structure of Insulin: Proinsulin – 3 peptides (A, B, C)

In mature Insulin: C removed. A & B remain
Company: Eli Lily
Technque: E. coli populations
1 with gene for A peptide
2 with gene for B peptide
separately formed A& B
isolated and joined together by disulfide bridges in vitro

  1. GENE THERAPY

Adenosine Deaminase deficiency (ADA deficiency)

ADA enzyme – for immune system

Disease due to defective Gene for Adenosine deaminase (ADA)

Problem: Lmphocyte not able to fight diseases (defective)

Cure:
  1. Bone marrow transplantation – not very successful. Possible in small children
  2. Enzyme replacement therapy –
lymphocyte from blood isolated                   cDNA containing unction ADA gene inserted (using retrovirus vector)                 functional lymphocyte returned to blood
Problem: Lymphocyte die. So need to be replaced periodically

Permanent cure: Genetic engineering of bone marrow cells (that form lymphocyte)

  1. Molecular Diagnosis

ELISA : enzyme linked immunosorbent assay
Based on antigen antibody interaction

PCR: can detect very low amount of DNA
a-1-antitrypsin: used to cure emphysema
Also cure for phenyl ketonuria & Cystic fibrosis
Transgnic Cow (Rosie) protein rich milk (2.4gm/l); human alpha lactalbumin enriched
Transgenic Animals: Advantages
  1. Study of normal physiology & development
  2. Study of disease
  3. Production of Biological products
  4. Vaccine safety testing
  5. Chemical safety testing
What is Biopiracy?
GEAC: Genetic Engineering Approval Committee: Indian committee for check on Genetic research
Indian patents Bill
Attempts at Biopiracy: Basmati Rice, Neem, Turmeric
SUMMARY:  Write 4 or more sentences describing specific learning from these notes.
__________________________________________________________________________________________
__________________________________________________________________________________________
__________________________________________________________________________________________
__________________________________________________________________________________________
__________________________________________________________________________________________

Thursday 11 January 2018

KEY POINTS ORGANISMS AND POPULATION PART 1

 XII

     Topic:  ORGANISM AND POPULATION 


QUESTIONS

NOTES


Ecology: study of interactions among org., n b/w its phy/abiotic env

4 levels: organisms <  population <  communities < biomes

At org level – physiological (adaptations for survival n reproduction) along course of evolution thru nat selection… adaptations developed

Biome formation: rotation of sun leading to temp variations, seasons, precipitation

Major Biomes: Desert, Grassland, Tropical, Temperate n Coniferous forests, Arctic n Alpine Tundra

Habitat: Regional & local variation

*Intestine (habitat for 100s of microbes)

Habitat determined by:


Temp: variations due to season, altitude, altitude.
            Range sub 0 - >50 deg C
Excep: thermal springs, deep sea hydrothermal vents (temp upto 100 deg C)

Impact of temp on:
  • enzyme Kinetics
  •  Basal Metabolism
  • Physiological functions

Sunday 18 December 2016

REPRODUCTIVE HEALTH



CHAPTER 4
REPRODUCTIVE HEALTH

WHO - WORLD HEALTH ORGANISATION definition
Health: Health is a total well-being in all aspects of reproduction, i.e., physical, emotional, behavioral & social; and not merely the absence of disease or infirmity.

Reproductive health is defined as a state of physical, mental, and social well-being in all matters relating to the reproductive system, at all stages of life; and not merely the absence of disease or infirmity.
Reproductive health addresses the reproductive processes, functions and system at all stages of life. It, therefore, implies that people are able to have a responsible, satisfying and safe sex life and that they have the capability to reproduce and the freedom to decide if, when and how often to do so.

Reproductive Health; Importance:
Reproductive health is a crucial part of general health and a central feature of human development.  It is a reflection of health during childhood, and crucial during adolescence and adulthood, sets the stage for health beyond the reproductive years for both women and men, and affects the health of the next generation.  The health of the newborn is largely a function of the mother's health and nutrition status and of her access to health care.
Factors affecting reproductive health
Reproductive Health– problems and strategy:

India was among the 1st countries to initiate actions & plans to attain total reproductive health as social goal.
These programmes are called as ‘FAMILY PLANNING’
Improved programmes currently in operation have a popular name ‘Reproductive & Child Health Care Programmes’ (RCH). Launched in 1977.

Sexual and Reproductive Health Awareness Day is held annually on February 12.

Problems:
·        Lack of awareness and readiness to discuss sex related issues
·        Myths and misconceptions related to sex
·        Early marriages and lack of prenatal and post natal care, leading to high maternal mortality
·        Unwanted or mistimed pregnancies
·        Prevalence of sexually transmitted diseases and reproductive tract infections
·        Population explosion
·        Illegal abortion of female fetuses
·        Congenital or acquired infertility

Aims of Reproductive Health Programme

·        To create awareness about various aspects of and related to reproduction
·        To educate adolescents regarding the significance of reproductive health, making the right choices and providing a healthy understanding of the sex related issues.
·        To lower the incidences of sexually transmitted diseases, and reproductive tract infections, by creating awareness regarding the significance of safe and hygienic sexual practices and proper care of reproductive organs.
·        To address issues of fertility, ensure counseling and guidance to couples incapable of bearing children. Development of assisted reproductive technologies (ARTs).
·        To educate regarding the importance of prenatal, and post natal care of mother and child.
·        To make people aware of legal issues related to reproductive process and technologies such as female foeticide and amniocentesis.
·        To develop technology for faster dissemination of knowledge and use of audio visual methods for developing a better understanding.

HOW HAS THE GOVERNMENT TAKEN MEASURES?

·        The use of audio-visuals & print media to create awareness
·        Parents, family members, close relations, teachers and friends are involved in the awareness programmes.
·        Introduction of Sex education in schools. Young students are most vulnerable to misconceptions and hence it was considered important to introduce sex education to them.
·        Proper information about reproductive organs, adolescence and related physiological changes, safe & hygienic sexual practices, sexually transmitted diseases, AIDS etc. 
·        Educating fertile couples, especially those of marriageable age group about available birth control options, proper care of pregnant mothers, pre and post natal care, importance of breast feeding etc.
·        Development of understanding of male and female physiology, their respective social and biological roles an in the light of this information; development of gender equality.
·        Awareness of problems related to population explosion, both at family and society level. Importance of small family, and limited resources.
·        Awareness of social evils and sex related crimes. Educating children to identify, and understand as well as protect themselves from these evils.
·        Development of infrastructure, technology and medical expertise to help people in need.
·        Research on reproduction related areas, to develop new method and improve existing methods.
·        Statutory ban on amniocentesis, massive child immunization programme etc.
·        Legalisation of MTP (medical termination of pregnancy) in 1971, to control population size by check unwanted pregnancies.
Overall development goal of measures taken by government is building up of a socially responsible and healthy society.

Amniocentesis
A fetal sex determination test based on the chromosomal pattern in the amniotic fluid surrounding the developing embryo.
It is used to find out chromosomal abnormalities in developing embryo by using amniotic fluid.
It is also illegally used for foetal sex determination and hence a statutory ban on amniocentesis has been placed to check female foeticide.

Indicators of Reproductive Health
·        Awareness of sex and reproduction related matters
·        Higher number of medically assisted deliveries
·        Better pre and post natal care, indicated by lower maternal and infant mortality
·        Smaller families with more couples opting for family planning programmes
·        Early detection and timely cure of STDs and other sex related problems
·        Higher number of ARTs, reducing problems of infertility

Population explosion & Birth control:
Scientific study of human population: Demography

 World population (1900): 2 billion                    India’s Population (1947): 350 million
World Population (2001): 6 billion                                India’s Population (2000): 1b

v  India is the second most populous country in the world
v  India’s population reached 1 billion on 11 may, 2000

Population Index:
Ø  Birth Rate (Natality rate): Natality rate is the rate at which new individuals are added to a particular population by reproduction (birth of young ones or hatching of eggs or germination of seeds/spores). It is generally expressed as number of births per 1,000 individuals of a population per year.
Ø  Death rate (Mortality rate): Mortality rate is the rate at which the individuals die or get killed. It is the opposite of natality rate. Mortality rate is generally expressed as number of deaths per 1,000 individuals of a population per year.
Ø  Vital Index: The percentage ratio of natality over mortality expressed in percentage is called Vital index.
Vital index determines the normal rate of growth of a population.

Ø  Growth Rate: The "population growth rate" is the rate at which the number of individuals in a population increases in a given time period. It is expressed as a fraction of the initial population.

Ø  Total fertility rate (TFR): TFR is defined as the average number of children that would be born to a woman in her lifetime if the age specific birth rates remain constant. The value of TFR varies from 1.9 in developed nations to 4.7 in developing nations. 


Causes of Population Explosion
·        Increased food production and distribution
·        Improvement in public health due to better sanitation, and cleaner water
·        Improvement in medical technology leading to conquest of diseases though early detection, better health practices, new medicines, vaccinations etc.
These factors have resulted in:
·        Decline in death rate
·        Decline in MMR (maternal mortality rate)
·        Decline in IMR (infant mortality rate)
·        Increase in number of people in reproductive age


According to the Census of 2001- 2011, India's population has grown at 17.7 per cent as against 21.5 per cent in the decade. At this rate it will double in next 33 years. This would lead to scarcity of basic requirements for life; food, shelter and clothing. Hence the government has been forced to take significant measures for population control.

Methods to Control population:
Ø  Education
Ø  Raising age of marriage
Ø  Family Planning methods

Steps Taken to execute the above:
·        Targeting an increase in literacy rate
·        Involving social organizations to make family planning programme a people’s programme
·        Providing education and employment opportunities to women
·        Proper implementation of community health programme
·        Incentives to people undergoing sterilization
·        Providing birth control facilities to individuals

CONTRACEPTIVE METHODS:

Through media – HUM DO HAMARE DO!!!!! (WE 2, OUR 2)
SAHELI - it is a contraceptive method developed by scientists in CDRI - Central Drug Research Institute.

Contraceptive Methods:

Natural Methods
Barriers
 I U D
Oral contraceptives
Injectable implants
Surgical Methods

1. Natural Methods:
Avoids  meeting of sperm & ovum.

Periodic Abstinence - Avoid coitus from day 10 – 17 of  menstrual cycle when ovulation is expected. Because chances of fertility is very high during this period, hence known as fertile period.
Withdrawal or coitus interuptus - Male partner withdraws his penis from vagina before ejaculation avoiding insemination of sperms
Lactational amenorrhea-Absence of menstrual cycle during first six months of intense lactational period.

2. Barrier Methods:

Condoms - Thin rubber used to cover penis in male or vagina & cervix in females.
Diaphragms, Cervical caps & vaults are all barriers for females to cover cervix during coitus.

Advantages of barrier methods:
1.       They are disposable.
2.       They can be self –inserted.
3.       They are reusable.
4.       Prevents conception by blocking entry of sperm thru cervix.
3. Intra uterine  devices (IUD’S):

Devices   inserted by doctors or nurses in uterus thru vagina.
Examples. Cu T, Cu7, Multiload 375, Lippes loop.

Cu ions released suppress sperm motility & fertilizing capacity of sperms.
Hormone releasing IUDs makes the uterus unsuitable for implantation & cervix hostile to the sperm.

4. Oral pills:

Pills are taken daily for 21 days.
They are very effective with less side effects.
Saheli - new oral contraceptive contains a non-steroidal preparation.
It is a ‘once a week ‘pill with high contraceptive value.
Injection or implantation of progesterone /estrogen under the skin.

5. Surgical  method:

This method is also called as STERILISATION.
It is advisable for male/female partner as a terminal method to prevent any more pregnancies.
In male, they’re called vasectomy, where the vas deferens is cut or tied.
In female, it is called tubectomy, where a small part of the fallopian tube is cut or tied up.
This method is highly effective but their reversibility is very poor.

Side effects of contraceptive method:
•       It is very important that the selection of contraceptive method should be taken under the consultation of the doctors.
•       However, their possible ill-effects like nausea, abdominal pain, breakthrough bleeding, irregular menstrual bleeding or even breast cancer.
•       These symptoms SHOULD NOT BE TOTALLY IGNORED.

What is MTP?
Intentional or voluntary termination of pregnancy before full term is called Medical Termination of Pregnancy (MTP) or Induced Abortion.

Why MTP?

MTP is done to get rid of unwanted pregnancies due to casual unprotected intercourse or failure of the contraceptive used during coitus or rape.
MTPs are also essential in certain cases where continuation in pregnancy could be harmful or even fatal to the mother or to the foetus or both.

Sexually transmitted diseases ( STDs)
Diseases or infections which are transmitted sexually through sexual intercourse are called as Sexually Transmitted Diseases (STDs) or Venereal Diseases (VDs) or reproductive tract infections. STDs can be classified as viral, bacterial, protozoan, fungal, etc.

HOW ARE STDS CAUSED?
Depending on the disease, STDs can be spread with any type of sexual activity. STDs are most often caused by viruses and bacteria.

Various types of Sexually Transmitted Diseases:
The various types of sexually transmitted diseases include gonorrhoea, syphilis, genital herpes, chancroid and of course the most common HIV leading to AIDS.

Chlamydiasis

Chlamydiasis is a sexually transmitted disease in humans caused by the bacterium Chlamydia trachomatis. Chlamydiasis is a major infectious cause of human genetial and eye diseases.
Chlamydiasis was once the most important cause of blindness. the infection can spread from eye to eye by fingers, shared towels, eye seeking flies, and cloths etc.

Prevention
STDs are a major threat to a healthy society. Therefore early detection or prevention and cure of these Diseases are given prime consideration under Reproductive health-care programmes though all person are vulnerable to these infections, their incidences are reported to be very high among the age group of 15-24years.these infections can be prevented by following a few simple rules which include:

Avoid sex with unknown partners or multiple partners
Always use condoms during coitus
In case of doubt, go to a qualified doctor for early detection and get complete treatment if diagnosed with disease.

INFERTILITY
A large no of couples all over India are infertile, i.e., they are unable to produce children in spite of Unprotected sexual co-habitation. The reasons for this could be many-physical, congenital, diseases, drugs, Immunological or even Psychological.

Assisted Reproductive Technologies (ART) are special techniques that assist couples to have children.
VARIOUS TYPES OF ASSISTED REPRODUCTIVE TECHNOLOGIES ( ART)INCLUDE:
·         In-vitro fertilisation (IVF)
·         Zygote intra fallopian transfer (ZIFT)
·         Intra cytoplasmic sperm injection(ICSI)
·         Gamete intra fallopian transfer(GIFT)
·         Artifical insemination (AI)

1) In Vitro Fertilization (IVF)
It is the fertilization outside the body in almost similar conditions as that in the body. In this method, popularly known as test tube baby programme, ova from the wife / donor (female) and sperms from the husband / donor (male) are collected and are induced to form the zygote under simulated conditions in the lab. The zygote or early embryos could then be transferred into the fallopian tube (ZIFT -zygote intra fallopian transfer)

2) Zygote intra fallopian transfer (ZIFT)
The zygote with 8 blastomeres can be transferred into the fallopian tube.

3) Intra cytoplasmic sperm injection (ICSI)
Intra cytoplasmic sperm injection (ICSI) is another specialized procedure to form an embryo in the lab in which a sperm is directly injected into the ovum.

4) Gamete intra fallopian tube (GIFT)
Transfer of an ovum collected from a donor into the fallopian tube of another female who cannot produce one, but can provide suitable environment for fertilisation and further development is another method attempted.

5) Artificial Insemination (AI)
Infertility cases either due to inability of the male partner to inseminate the female or due to very low sperms counts in the ejaculates could be corrected by artificial insemination.

In the technique, the semen collected either from the husband or a healthy donor is artificially introduced into the vagina or into the uterus (IUI - Intra Uterine Insemination) of the female.

6) Adoption – can be done from orphanage / relatives.

Counseling and information on infertility

It is important to involve both partners in all aspects of management. Discussions of wishes, plans, beliefs and motives are important.